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KMID : 1234420100380010105
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Korean Journal of Microbiololgy and Biotechnology
2010 Volume.38 No. 1 p.105 ~ p.111
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Antibacterial Activities of Caesalpinia sappan L. Extract and Structural Analysis of Its Related Brazilin
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Kwon Hyun-Jung
Kim Young-Hyun
Nam Kung-Woo
Kim Sun-Ki
Bang In-Soek
Han Man-Deuk
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Abstract
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Caesalpinia sappan L. has long been commonly used in oriental folk medicines to treat diseases. To investigate the antibacterial effects from C. sappan L. heart wood, the MeOH soluble extract was successively fractionated by using hexane, CHCl3, EtOAc, BuOH, MeOH, and H2O. Among of these extracts, the EtOAc fraction which partitioned to 3.94% of the highest yields was to be the most active against all human pathogenic bacteria in this experiment. In addition, the antibacterial activities of the EtOAc fraction were more effective against Gram (+) bacteria compared to those against Gram (-) bacteria, which showed difference of the antibacterial activities against Gram (-) bacteria. To confirm the identity of the active substances, the EtOAc fraction was further separated by silica gel adsorption column, high performance liquid chromatography, and 98.48% purity of brazilin (1.67 mg)/ EtOAc (10 mg) fraction was obtained from 300 g of C. sappan L. heart wood. The isolated active substance was a single compound of yellow crystalline, and was identified as brazilin (C16H14O5) by MS, and 1H-NMR and 13C-NMR. These results suggest that the brazilin in the EtOAc fraction from MeOH extract of C. sappan L. has a potential as a natural therapeutic agent against human pathogenic Gram (+) bacteria such as Staphylococcus aureus.
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KEYWORD
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Caesalpinia sappan L., ethyl acetate fraction, antibacterial activity, brazilin, structural analysis
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